Isolation and Characterization of Four Gram-Positive Nickel-Tolerant Microorganisms from Contaminated Sediments
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Joy D. Van Nostrand [1] ; Tatiana V. Khijniak [1] ; Terry J. Gentry [2] ; Michelle T. Novak [1] ; Andrew G. Sowder [3] ; Jizhong Z. Zhou [2] [4] ; Paul M. Bertsch [1] [3] ; Pamela J. Morris [1] [5]
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[1] Medical University of South Carolina
Medical University of South Carolina
Estados Unidos
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[2] Oak Ridge National Laboratory
Oak Ridge National Laboratory
Estados Unidos
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[3] University of Georgia
University of Georgia
Estados Unidos
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[4] University of Oklahoma
University of Oklahoma
Estados Unidos
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[5] National Oceanic and Atmospheric Administration
National Oceanic and Atmospheric Administration
Estados Unidos
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- Localización: Microbial ecology, ISSN-e 1432-184X, ISSN 0095-3628, Vol. 53, Nº. 4, 2007, págs. 670-682
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Microbial communities from riparian sediments contaminated with high levels of Ni and U were examined for metal-tolerant microorganisms. Isolation of four aerobic Ni-tolerant, Gram-positive heterotrophic bacteria indicated selection pressure from Ni. These isolates were identified as Arthrobacter oxydans NR-1, Streptomyces galbus NR-2, Streptomyces aureofaciens NR-3, and Kitasatospora cystarginea NR-4 based on partial 16S rDNA sequences. A functional gene microarray containing gene probes for functions associated with biogeochemical cycling, metal homeostasis, and organic contaminant degradation showed little overlap among the four isolates. Fifteen of the genes were detected in all four isolates with only two of these related to metal resistance, specifically to tellurium. Each of the four isolates also displayed resistance to at least one of six antibiotics tested, with resistance to kanamycin, gentamycin, and ciprofloxacin observed in at least two of the isolates. Further characterization of S. aureofaciens NR-3 and K. cystarginea NR-4 demonstrated that both isolates expressed Ni tolerance constitutively. In addition, both were able to grow in higher concentrations of Ni at pH 6 as compared with pH 7 (42.6 and 8.5 mM Ni at pH 6 and 7, respectively). Tolerance to Cd, Co, and Zn was also examined in these two isolates; a similar pH-dependent metal tolerance was observed when grown with Co and Zn. Neither isolate was tolerant to Cd. These findings suggest that Ni is exerting a selection pressure at this site for metal-resistant actinomycetes.
