Histochemial study of lymphocystis disease in skin of gilthead seabream, Sparus aurata L.

Sarasquete, C. ^[1] ; Gonzalez de Canales, M. L. ^[2] ; Arellano, J. ^[1] ; Pérez Prieto, S. ^[3] ; García Rosado, E. ^[4] ; Borrego, J. J. ^[4]
1. [1] Instituto de Ciencias Marinas de Andalucía
  
  Instituto de Ciencias Marinas de Andalucía
  
  Cádiz, España
2. [2] Universidad de Cádiz
  
  Universidad de Cádiz
  
  Cádiz, España
3. [3] Centro de Investigaciones Biológicas
  
  Centro de Investigaciones Biológicas
  
  Madrid, España
4. [4] Departamento de Microbiologia, Facultad de Ciencias, Campus Teatinos, Malaga, Spain
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Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 13, Nº. 1, 1998, págs. 37-45
Idioma: inglés
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Resumen
- A battery of horseradish peroxidaseconjugated lectins (Con A. WGA and DBA). as well as conventional histochemical techniques (PAS.
  
  saponification , Alcian Blue pH 0.1. I. 2.5. chlorhydric hydrolisis. sialidase, Bromophenol blue. Tioglycollate reduction and Ferric-ferricyanide-FeIll) were used to study the content and distribution of carbohydrates, proteins and glycoconjugatc sugar residues on the skin and on the Iymphocystis-infected cells of gilthead seabrcam, SpanlS aurota. Variable amounts of glycoproteins containing sialic acid. N-acetyl-Dglucosamine , N-acetyl-D-galactosamine. mannose and/or glucose residues were observed in the cuticle and mucous cells of the corporal skin. tails and fins.
  
  Germinative and epithelial cells of the epidermis contained glycogen. proteins. carboxylated groups. as well as glycoproteins with mannose and/or glucose and N-acetyl-D-galactosamine residues. Hyaline capsule of the mature Iymphocystis-infected cells was strongly stained with PAS. Alcian Blue (pH 0.5 and 2.5) and weakly positive with Alcian Blue (pH I). Con A reacted with the granular cytoplasm, specially around hyaline capsule. and with the basophilic intracytoplasmic inclusions developed in mature Iymphocystis-infected cells of Spartls aurota skin. These sugar residues (mannose and/or glucose), as well as N-acetyl-Dglucosamine and /or sialic acid and N-acetyl-Dgalactosamine were not detected in the hyaline capsule of the Iyr,lphocystis disease.