Dynamics of Sulfate-Reducing Microorganisms ("dsrAB" genes) in Two Contrasting Mudflats of the Seine Estuary (France)
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J. Leloup [1] [2] ; F. Petit [2] ; D. Boust [3] ; J. Deloffre [2] ; G. Bally [2] ; O. Clarisse [4] ; L. Quillet [2]
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[1] Max Planck Institute for Marine Microbiology
Max Planck Institute for Marine Microbiology
Kreisfreie Stadt Bremen, Alemania
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[2] University of Rouen
University of Rouen
Arrondissement de Rouen, Francia
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[3] Laboratoire d'études radioécologiques de la façade Atlantique, IRSN, Cherbourg-Octeville, France
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[4] Laboratoire de Chimie Analytique Marine, UMR CNRS 8013, Université des Sciences et Technologies de Lille, France
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- Localización: Microbial ecology, ISSN-e 1432-184X, ISSN 0095-3628, Vol. 50, Nº. 3, 2005, págs. 307-314
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
By combining molecular biology and biochemical approaches, the dynamics of sulfate-reducing microorganisms (SRM) was investigated in the sediments of the Seine estuary (France). Both intertidal mixing-zone and freshwater mudflats were sampled during a 1-year period; the quantification of SRM was realized by using competitive polymerase chain reaction (PCR) based on dsrAB gene amplification, previously described by Leloup et al. (2004), and sulfate reduction rate (SRR) was determined via the SO4 2 radiotracer method. Throughout the year, abundance of dsrAB genes and SRR were predominantly high in the top 15 cm of the sediment. A seasonal dynamic was observed; a predominance of activity was noted during the early summer, and seems to be mainly controlled by physical–chemical parameters (temperature and dissolved organic carbon concentration) and topographic evolution of the mudflat (erosion/deposit erosion).
