Development of a qPCR assay for the detection of heat-resistant Talaromyces flavus
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Jacek Panek [1] ; Magdalena Frąc [1]
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[1] Institute of Agrophysics
Institute of Agrophysics
Lublin, Polonia
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- Localización: International journal of food microbiology, ISSN 0168-1605, Vol. 270, Nº. 1, 2018, págs. 44-51
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Heat-resistant fungi of the species Talaromyces flavus, which inhabits soil and can contaminate fruits, constitutes significant impact on spoilage of heat-processed food. T. flavus possess the ability to produce numerous mycotoxins and is able to survive the process of pasteurization what makes it a treat to food industry. Up to date there is no rapid and reliable method to detect and identify T. flavus. Therefore in this study, a sensitive method for detecting T. flavus was developed. The primers (Tf1_F/R) specific to detection of DNA replication licensing factor gene of T. flavus were designed. With this set of primers, a qPCR reaction with SybrGreen detection was developed. The specificity of assay with use of 5 T. flavus strains and 35 other fungal isolates was tested. The detection threshold was 200 fg of T. flavus genomic DNA. The developed method was able to detect 640 ascospores in 1 g of strawberry fruits and soil samples.
