Identification of Diazotrophs in the Culturable Bacterial Community Associated with Roots of "Lasiurus sindicus", a Perennial Grass of Thar Desert, India

• Soumitra Paul Chowdhury ^[1] ; Michael Schmid ^[2] ; Anton Hartmann ^[2] ; Anil Kumar Tripathi ^[1]
  1. [1] Banaras Hindu University

     Banaras Hindu University

     India

     
  2. [2] Department of Microbe-Plant-Interactions, GSF-National Research Centre for Environment and Health, Neuherberg/Munich, Germany
• Localización: Microbial ecology, ISSN-e 1432-184X, ISSN 0095-3628, Vol. 54, Nº. 1, 2007, págs. 82-90
• Idioma: inglés
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• Resumen

  • Lasiurus sindicus is a highly nutritive, drought-tolerant, perennial grass that is endemic to the Thar Desert of Rajasthan, India. Analysis of 16S rRNA coding genes of the bacterial isolates enriched in nitrogen-free semisolid medium, from the surface-sterilized roots of L. sindicus, showed predominance of Gram-negative over Gram-positive bacteria. According to comparative sequence analysis of 16S rDNA sequence data, Gram-positive bacteria with low GC content (Staphylococcus warneri and Bacillus sp.) and high GC content (Micrococcus luteus, Microbacterium sp.) were identified. Gram-negative bacteria included Azospirillum sp., Rhizobium sp., Agrobacterium tumefaciens, and Inquilinus limosus (α-proteobacteria); Ralstonia sp., Variovorax paradoxus, and Bordetella petrii (β-proteobacteria); and Pseudomonas pseudoalcaligenes, Stenotrophomonas sp. (γ-proteobacteria). The occurrence of nifH sequences in Azospirillum sp., Rhizobium sp., and P. pseudoalcaligenes showed the possibility of supplying biologically fixed nitrogen by the root-associated diazotrophs to the host plant.