The use of fluorescent dextrans as a marker of sarcolemmal injury

• Carter, G.T. ^[1] ; Kikuchi, N. ^[1] ; Horasek, S.J. ^[1] ; Walsh, S.A. ^[1]
  1. [1] University of California System

     University of California System

     Estados Unidos

     
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 9, Nº. 3, 1994, págs. 443-447
• Idioma: inglés
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  • We investigated the use of intravenously injected fluorescent dextran molecules (FDx) as a histological marker of sarcolemmal injury. Using fluorescent microscopy, uptake of FDx (average MW 10 kD) was assessed in sections of quadriceps muscles from three models: l ) normal (C57BLlIOSnJ) mice. 2) normal mice run downhill (0, 3, and 7 days post exercise), and 3) non-exercised mdx (dystrophin-deficient) mice. These were compared to serial sections stained with hematoxylin and eosin (H&E). In control muscles. strong fluorescence was seen between fibers (intercellular). Intracellular FDx was observed within cells of the quadriceps from normal mice run downhill at days 0 and 3 post exercise, but not at day 7. On H&E staining. muscle pathology was not observed until day 3, with regeneration by day 7. Intracellular FDx was also observed within mdx muscles, particularly in fibers that appeared pre-necrotic on H&E stained sections. FDx appears to be useful as a histological marker of changes in sarcolemmal integrity associated with muscle injury from eccentric exercise or muscle disease.