Does diabetic state affect co-localization of peptide YY and enteroglucagon in colonic endocrine cells?

• A. Spångéus ^[1] ; S. Forsgren ^[1] ; M. EI-Salhy ^[1]
  1. [1] University Hospital,Umefi, Sweden
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 15, Nº. 1, 2000, págs. 37-41
• Idioma: inglés
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  • Background: Changes in the numbers of pyy- and enteroglucagon-immunoreactive cells in colon of animal models of human diabetes have been reported.

    As these peptides co-localize in the same cells it is possible that the observed changes are a result of changes in co-localization. Methods: Animal models of human type 1 and type 2 diabetes, namely the non-obese diabetic (NOD) mouse and the obese (ob/ob) mouse, were studied. As controls for the NOD mice, BALB/cJ mice were used and for ob/ob mice, homozygous lean (+/+) mice were used. Tissue samples from colon were double-immunostained for PYY and enteroglucagon according to the indirect immunofluorescence method.

    Results: Co-localization of enteroglucagon and PYY was found in colonic endocrine cells in all grou ps investigated. Compared with controls, pre-diabetic NOD mice showed a decreased proportion of enteroglucagon/PYY co-localization. There was no difference in diabetic NOD mice or diabetic ob/ob mice when compared with controls. Conclusions: Whereas the number of cells containing solely enteroglucagon and solely PYY increases in pre-diabetic NOD mice, production of enteroglucagon in PYY -immunoreactive cells decreases. Although the numbers of PYY and enteroglucagon cells have been reported to be changed in both diabetic NOD mice and in obese mice , the balance between co-expressing and mono-expressing cells seems to be preserved.