Human papillomaviruses and DNA ploidy in anal condylomata acuminata

• S. Rlhet ^[1] ; P. Bellalch ^[1] ; M. Lorenzato ^[1] ; D. Bouttens ^[1] ; P. Bernard ^[1] ; P. Birembaut ^[1] ; C. Clavel ^[1]
  1. [1] C.H.U. de Reims, France
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 15, Nº. 1, 2000, págs. 79-84
• Idioma: inglés
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• Resumen

  • Previous studie s have emphasized the usefulness of DNA ploidy measurement and Human Papiliomavirus (HPY) detection as pronostic markers in low grade cervical lesions. We addressed the eventual relationship between HPY type, DNA profile, and p53 tumor suppressor protein expression in anal condylomata acumi nata to eventually determine parameters which may be considered as predictive risk factors for the development of cancer. DNA ploidy was assessed by image cytometry after Feulgen stai ning of contiguous serial sections of 45 anal condylomata acuminata without atypia containing HPY detected by in situ hybridization and Polymerase Chain Reaction (PCR).

    p53 expression was detected by immunohistochemistry.

    DNA aneuploidy was found in 53.3% of these lesions, 48.9% containing non oncogenic HPY types 6 and/or 11 and 4.4% harbouring HPY types II and 18. The DNA diploid lesions were all associated with non oncogenic HPY types 6 and/or 11 and one case also contained HPY type 33. There was no significant correlation between the detection of DNA aneuploidy and the presence of immunodetected p53. DNA aneuploidy was not related to the presence of oncogenic HPY in anal condylomata acuminata. The DNA aneuploid profile frequently observed, especially in lesions associated with non oncogenic HPY types, is not yet well explained and cannot be considered as a prognostic factor. In contrast, a more intensive clinical follow-up should be proposed in patients with oncogenic HPY associated to DNA aneuploidy.