Morphometric evidence of the regulation of the activity of LH-immunoreactive cells by corticosterone. An in vivo and in vitro study
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Rubio, M. [1] ; Carretero, J. [1] ; Cabo, J.J. [1] ; Santos, M. [1] ; Vázquez, R. [1] ; Sánchez, F. [1]
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[1] Universidad de Salamanca
Universidad de Salamanca
Salamanca, España
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- Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 11, Nº. 4, 1996, págs. 931-935
- Idioma: inglés
- Enlaces
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Resumen
The present study was performed to investigate the morphometric effects of the administration of corticosterone on LH-immunoreactive cells of pituitary monolayer cultures. In addition, the effect of bilateral adrenalectomy on the LH-adenohypophyseal cells was explored. In vitro administration of corticosterone induced a marked decrease in the morphometric parameters considered (cellular, cytoplasmic and nuclear areas). In vivo, bilateral adrenalectomy produced a significant increase in these parameters when compared to those obtained in normal and sham-operated animals. However, when the bilaterally adrenalectomized rats received a daily dose of corticosterone no significant changes in the morphometric parameters were found. Morphometrically, these combined in vivo-in vitro findings confirm a corticosterone-induced hypoactivity of LI4-cells together with a direct effect of this glucocorticoid on LH-adenohypophyseal cells.
