Development and progression of malignancy in human colon tissues are correlated with expression of specific Ca2+-binding S100 proteins

• Bronckart, Y. ^[2] ; Decaestecker, C. ^[2] ; Nagy, N. ^[2] ; Harper, L. ^[2] ; Schafer, B.W. ^[1] ; Salmon, I. ^[2] ; Pochet, R. ^[2] ; Kiss, R. ^[2] ; Heizman, C.W. ^[1]
  1. [1] University of Zurich

     University of Zurich

     Zürich, Suiza

     
  2. [2] Eramus University Hospital, Free University of Brussels (ULB); Brussels, Belgium
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 16, Nº. 3, 2001, págs. 707-712
• Idioma: inglés
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  • The expression levels of seven different SlOO proteins (SlOOAl, SlOOA2, SlOOA3, SlOOA4, SlOOA5, SlOOA6 , and SlOOB) were characterized by immunohistochemistry in the epithelial versus connective tissues of a series of 35 colon specimens, including 6 normal samples, 5 adenomas with low-grade dysplasia, 5 adenomas with high-grade dysplasia, and 19 cancers. The results showed that SlOOA2, SlOOA3, and SlOOB proteins could not (or only marginally) be detected in colon tissues. On the other hand , th e expression of SlOOA6 increased in epithelial tissues directly proportional to the increase of malignancy. The percentage of epithelial (or connective tissue) cells expressing SlOOA4 significantly decreased as the malignancy grade increased. The expression level of SlOOAl proteins was somewhat higher in the connective tissues of normal cases and adenomas with low-grade dysplasia than in adenomas with high-grade dysplasia and cancers. This pattern of expression was not observed in epithelial tissues. While the node-positive cancers did not express SlOOAl, about half of the node-negative specimens did . The expression levels of SlOOA5 were similar in different epithe lial tissues. However, in the connective tissues the expression levels decreased inversely proportional to the increase in pathological grading of the specimens. Therefore, the present study implicates several SlOO proteins as useful too ls for histochemical typing of colon cancer malignancy development.