Aqueous peel extract of Citrus aurantifolia attenuated tramadol-mediated testicular oxidative stress, apoptosis, inflammations and histo-architectural degeneration in a rat model

• Gabriel Akunna ; Philip Ihechu ^[1] ; Akinola Ajeleti ^[1] ; Olaleye Olabiyi ^[1] ; Ademola Famurewa ^[2] ; Linus A. Enye ; Amos Morakinyo ^[1]
  1. [1] Department of Anatomy, Faculty of Basic Medical Sciences, College of Medicine and Health Sciences, Bowen University, Iwo, Osun State
  2. [2] Department of Anatomy, College of Medicine and Health Sciences, Alex Ekwueme Federal University, Ebonyi State
• Localización: European Journal of anatomy, ISSN-e 1136-4890, Vol. 24, Nº. 5, 2020, págs. 429-437
• Idioma: inglés
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• Resumen

  • Tramadol is one of the most commonly abused drugs. It has been reported to impair testicular function in models. Citrus aurantifolia is a citrus fruit used extensively in beverages and herbal preparations. This study investigated the modulat-ing role of aqueous zest extract of Citrus aurantifo-lia (AZECA) against tramadol-mediated testicular toxicity in rat. Twenty-five male rats were assigned to 5 groups and orally treated with distilled water (negative control) for 28 days, 30mg/kg of tra-madol (positive control) for 14 days, 30 mg/kg of tramadol for first two weeks and 50 (treatment 1) and 100 mg/kg (treatment 1) of AZECA for the last 2 weeks. Biochemical markers were evaluated and testes were stained routinely for morphometry. Results shows a significant (p<0.05) decrease in testosterone, FSH and LH, SOD, CAT and a signif-icant increase MDA, IL-1 and TNF-∝ evidenced by a significant (p<0.05) reduction in volumetric proportion of interstitium and seminiferous tubules-tunica propria, macrophages, blood vessels and lymphatic space, macrophages, connective tissue, diameter, cross-sectional area and number of pro-files of seminiferous tubules per unit area in group treated with tramadol-alone. These parameters were significantly (p<0.05) negated in groups that had AZECA supplementation. This study has demonstrated that treatment with AZECA exerted a potent testiculo-protective activity against tra-madol-induced testicular injury in rats.