Expression and DNA methylation profiles of EZH2‑target genes in plasma exosomes and matched primary tumor tissues of the patients with diffuse large B‑cell lymphoma

I.C. Barıs; S. Hacıoglu; N.S. Turk; G.O. Cetın; S. Zencır; G. Bagcı; V. Caner

Ayuda

Expression and DNA methylation profiles of EZH2‑target genes in plasma exosomes and matched primary tumor tissues of the patients with diffuse large B‑cell lymphoma

I. C. Barıs ^[1] ; S. Hacıoglu ^[1] ; N. S. Turk ^[1] ; G. O. Cetın ^[1] ; S. Zencır ^[2] ; G. Bagcı ^[1] ; V. Caner ^[1]
1. [1] Pamukkale University
  
  Pamukkale University
  
  Turquía
2. [2] Université de Genève
  
  Université de Genève
  
  Genève, Suiza
Localización: Clinical & translational oncology, ISSN 1699-048X, Vol. 23, Nº. 6, 2021, págs. 1152-1166
Idioma: inglés
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Resumen
- Aims Diffuse large B-cell lymphoma (DLBCL) is the most common type of aggressive lymphoma. This study was designed to compare epigenetic alterations observed in Enhancer of Zeste Homolog 2 (EZH2)-target genes between plasma-derived exosomes and primary tumors in DLBCL patients.Main methodsExosomes were isolated from plasma of 21 DLBCL patients and 21 controls. We analyzed the methylation status of the target genes using methylation-specific PCR. We also examined whether the exosomes and the tumor samples contained transcripts of the target genes.Key findingsWe found that CDKN2A and CDKN2B were methylated in both plasma exosomes and primary tumor tissue samples. None of the transcripts were found in the exosomes except CDKN1B which was expressed in 8 (38%) of the exo-some samples.SignificanceThis study showed that plasma exosomes might preferably package certain target molecules from primary tumors and the exosomes containing dual methylated DNAs of CDKN2A and CDKN2B, or CDKN1B transcript may con-tribute to DLBCL pathogenesis.