Circulating MicroRNA Profiling Reveals Specific Subsignatures in Response to a Maximal Incremental Exercise Test
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Manuel Fernández Sanjurjo ; Ángel Enrique Díaz Martínez ; Sergio Díez Robles ; Felipe González González [1] ; David de Gonzalo Calvo ; Manuel Rabadán Ruiz ; Alberto Dávalos ; Benjamín Fernández García ; Eduardo Iglesias Gutiérrez
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[1] Universidad de Oviedo
Universidad de Oviedo
Oviedo, España
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- Localización: Journal of strength and conditioning research: the research journal of the NSCA, ISSN 1064-8011, Vol. 35, Nº. 2, 2021, págs. 287-291
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Circulating microRNAs (c-miRNAs) have been described as emergent regulators and biomarkers of exercise. The aim of this study was to analyze the c-miRNA response to a maximal incremental exercise test (MIET) and its relationship with markers of exercise response and adaptation. Two blood samples were collected from 9 male amateur runners (31-50 years), before (Pre) and after (Post) a MIET. The maximal oxygen uptake (V[Combining Dot Above]O2max), maximum heart rate (HRmax), and maximal aerobic speed (MAS) were recorded. Lactate and creatine kinase (CK) plasma concentrations were measured. A panel of 752 miRNAs was analyzed using standardized protocols and relative quantification to Pre. A total of 13 miRNAs were found significantly upregulated at Post. By focusing on the exercise markers that correlate with the expression of these miRNAs, they were clustered into different functional groups or subsignatures. Thus, miR-21-5p, miR-29b-3p, and miR-183-5p showed a strong correlation with HRmax and a validated target signature related to fatty acid metabolism. Furthermore, let-7c-5p, miR-340-5p, miR-425-3p, and miR-629-5p were significantly correlated with CK, and the most significantly enriched pathways for these subsignatures were the Hippo signaling pathway and signaling pathways regulating pluripotency of stem cells. Finally, Pre miR-106b-5p expression showed an inverse association with MAS and Post lactate concentration, which highlights its relevance as biomarker of training status and its predictive value for performance. No significant correlations were observed with V[Combining Dot Above]O2max. Our results define for the first time specific functional c-miRNA subsignatures, adding novel evidence about their potential regulatory role in exercise response.
