A comprehensive analysis of tumor microenvironment-related genes in colon cancer
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[1] Sixth Affiliated Hospital of Sun Yat-sen University
Sixth Affiliated Hospital of Sun Yat-sen University
China
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- Localización: Clinical & translational oncology, ISSN 1699-048X, Vol. 23, Nº. 9, 2021, págs. 1769-1781
- Idioma: inglés
- Enlaces
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Resumen
Background The development and progression of colon cancer are significantly affected by the tumor microenvironment, which has attracted much attention. The goal of our study was primarily to find out all possible tumor microenvironment- related genes in colon cancer.
Method This study quantified the immune and stromal landscape using the ESTIMATION algorithm using the gene expres- sion matrix obtained from the UCSC Xena database. Dysregulated genes were harvested using the limma R package, and relevant pathways and biofunctions were identified using enrichment analysis. A least absolute shrinkage and selection operator (LASSO) regression was used to select the pivotal genes from the DEGs. Then, survival analysis was performed to determine the hub genes and a prognostic model was constructed by these hub genes with (or) TNM stage. Besides, associa- tions between hub gene expressions and immune cell infiltration were assessed.
Results A total of 725 DEGs were identified. Most of the results of the enrichment analysis were immune-related items.
13 genes were selected as the hub genes and a moderate-to-strong positive correlation between most hub genes and several immune cells were observed. Besides, the prognostic value of the hub genes were comparable to TNM staging.
Conclusions Our study provides a better understanding of how interactions between the 13 immune-prognostic hub genes and immune cells in the tumor microenvironment affect biological processes in colon cancer. These genes exhibit an equivalent ability to TNM staging in prognosis prediction. They are particularly expected to become novel prognostic biomarkers and targets of immunotherapies for colon cancer
