MicroRNA‑802 promotes the progression of osteosarcoma through targeting p27 and activating PI3K/AKT pathway

• L. F. Gao ^[1] ; S. Jia ^[2] ; Q. M. Zhang ^[3] ; Y. F. Xia ^[4] ; C. J. Li ^[4] ; Y. H. Li ^[5]
  1. [1] Department of Clinical Laboratory, Weifang Weiyi Tumor Hospital, Affiliated Hospital of Weifang Medical University, Weifang 261061, China
  2. [2] No.1 Department of Orthopedics, Traditional Chinese Medical Hospital of Huangdao District, Qingdao 266500, China
  3. [3] Emergency Ward, Qingdao Hospital of Traditional Chinese Medicine, Qingdao Hiser Medical Group, Qingdao 266033, China
  4. [4] Department of Imaging, Zhangqiu District People’s Hospital, Jinan 250200, China
  5. [5] Department of Spine Surgery, Qingdao Hospital of Traditional Chinese Medicine, Qingdao Hiser Medical Group, 4 Renmin Road, Shibei District, Qingdao 266033, China

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• Localización: Clinical & translational oncology, ISSN 1699-048X, Vol. 24, Nº. 2, 2022, págs. 266-275
• Idioma: inglés
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• Resumen

  • Purpose Increasing evidences suggest dysfunctions of microRNAs (miRNAs) are playing important part in tumors. There- fore, the role of miR-802 in osteosarcoma (OS) was exploited. The object was to evaluate the effect of miR-802 and verify its influence on p27 Kip1 (p27) in OS.

    Methods RT-qPCR experiment was used to detect miR-802 and p27 expression in OS tissues and cells. We explored the function of miR-802 through Transwell assays. The phosphoinositide 3-kinase (PI3K)/AKT serine/threonine kinase pathway and epithelial–mesenchymal transition (EMT) was detected by Western blot assays. Luciferase assay was used to testify the target of miR-802.

    Results MiR-802 expression was elevated in OS, which was related to poor clinical outcome in OS patients. MiR-802 over- expression promoted OS migration, invasion and EMT. Further, p27 is a direct target of miR-802. P27 elevation counteracted the promotion effect of OS on EMT, migration and invasion induced by miR-802. In addition, miR-802 overexpression inactivated PI3K/AKT pathway via targeting p27 in OS.

    Conclusion MiR-802 promoted the progress of EMT, migration and invasion in OS via targeting p27. This newly identified miR-802/p27/PI3K/AKT axis may represent potential targets for OS.