Curcumol inhibits PDGF-BB-induced proliferation and migration of airway smooth muscle cells by suppressing ERK/CREB pathway

• Shihui Ling ^[1] ; Liya Zhang ^[1] ; Yan Qian ^[2] ; Zhiguang Liu ^[2] ; Zhengdao Mao ^[2] ; Qian Zhang ^[2]
  1. [1] Department of Emergency, The Affiliated Changzhou No.2, People’s Hospital of Nanjing Medical University, Changzhou City, Jiangsu Province, China.
  2. [2] Department of Respiratory and Critical Care Medicine, The Affiliated Changzhou No.2, People’s Hospital of Nanjing Medical University, Changzhou City, Jiangsu Province, China.
• Localización: Allergologia et immunopathologia: International journal for clinical and investigate allergology and clinical immunology, ISSN-e 1578-1267, ISSN 0301-0546, Vol. 50, Nº. 1, 2022, págs. 17-24
• Idioma: inglés
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• Resumen

  • Background: Curcumol, possessing antiviral, antifungal, antimicrobial, anticancer, and anti-inflammatory properties, has been widely used in treating cancers and liver fibrosis. The aim of this study was to determine the effect of curcumol on the progression of asthma.

    Materials and methods: Curcumol was administrated to platelet-derived growth factor (PDGF)-BB-stimulated airway smooth muscle cells (ASMCs). The proliferation of ASMCs was assessed by MTT and EdU incorporation assays. The apoptosis of ASMCs was measured by flow cytometry and Western blotting. The migration of ASMCs was evaluated by Transwell migration assay and Western blotting. The regulatory effects of curcumol on extracellular signal-regulated protein kinase (ERK)/cAMP response element-binding protein (CREB) pathway was evaluated by Western blotting.

    Results: The proliferation and migration of ASMCs induced by PDGF-BB was suppressed, and the apoptosis of ASMCs was elevated by curcumol in a dose-dependent manner. The activation of ERK/CREB pathway induced by PDGF-BB was suppressed by curcumol.

    Conclusion: Curcumol could suppress ERK/CREB pathway to inhibit proliferation and migration and promote apoptosis of PDGF-BB-stimulated ASMCs. These findings suggest that curcumol may act as a potential drug for asthma treatment.