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El objetivo fue determinar si una solución salina ozonizada (SSO3) incrementa los neutrófilos polimorfonucleares (NPMN) endometriales (Exp 1) y desafiar el efecto preventivo de SSO3 sobre la endometritis subclínica (ESC) (Exp 2). En el Exp 1 se utilizaron 38 vacas Holstein primíparas. Se incluyeron vacas con (CAM) y sin antecedentes de metritis postparto (SAM); posteriormente se repartieron en subgrupos control (CTRL, solución salina) o SSO3 (6.7 ± 0.3 ppm). A los 55 días postparto se aplicó 50 ml de CTRL o SSO3 intrauterinamente y a las 48 h se registró la cantidad de NPMN por citología endometrial. En el Exp 2 se utilizaron 26 vacas Holstein primíparas SAM. Las vacas se repartieron aleatoriamente en CTRL o SSO3. Se administraron dos dosis de 50 ml con intervalo de 7 días (primera aplicación 11.3 ± 0.4 días postparto). Al día 30 postparto se diagnosticó ESC (≥6% NPMN). Las vacas CAM tuvieron mayor número de NPMN endometriales comparado con vacas SAM (13.9 ± 6.2 vs 1.0±0.46, P<0.05). El grupo CAM-CTRL presentó mayor número de NPMN que el SAM-CTRL (17.0 ± 9.6 vs 0.1 ± 0.1, P<0.05) mientras que los grupos CAM-SSO3 y SAM-SSO3 (10.4 ± 8.1 vs 1.8±4.8, P>0.05) y SAM-CTRL y SAM-SSO3 (0.1 ± 0.1 y 1.8 ± 4.8, P>0.05) no fueron diferentes. Se observó una tendencia estadística (P=0.09) de menor porcentaje de ESC en grupo CTRL comparado con SSO3 (15.4 y 46.2 %, respectivamente). En conclusión, la SSO3 por vía transcervical no incrementa el número de NPMN endometriales y el tratamiento preventivo con SSO3 aplicado a vacas SAM no disminuyó la ESC. Los resultados sugieren un posible efecto antiinflamatorio del tratamiento solución salina ionizada.
The objective was to determine whether an ozonized saline solution (O3SS) increases endometrial polymorphonuclear neutrophils (PMNN) (Exp 1) and to challenge the preventive effect of O3SS on subclinical endometritis (SCE) (Exp 2). In Exp 1, 38 primiparous Holstein cows were used. Cows with (WHM) and no history of postpartum metritis (NHM) were included; they were then divided into control (CTRL, saline solution) or O3SS (6.7 ± 0.3 ppm) subgroups. At 55 d postpartum, 50 ml of CTRL or O3SS was applied intrauterinely and at 48 h, the quantity of PMNN was recorded by endometrial cytology. In Exp 2, 26 primiparous NHM Holstein cows were used. The cows were randomly distributed in CTRL or O3SS. Two doses of 50 ml were administered with an interval of 7 d (first application 11.3 ± 0.4 d postpartum). At d 30 postpartum, SCE (≥6 % PMNN) was diagnosed. WHM cows had higher numbers of endometrial PMNN compared to NHM cows (13.9 ± 6.2 vs 1.0 ± 0.46, P<0.05). The WHM-CTRL group had a higher number of PMNN than NHM-CTRL (17.0 ± 9.6 vs 0.1 ± 0.1, P<0.05), while the WHM-O3SS and NHM-O3SS groups (10.4 ± 8.1 vs 1.8 ± 4.8, P>0.05) and the NHM-CTRL and NHM-O3SS groups (0.1 ± 0.1 and 1.8 ± 4.8, P>0.05) were not different. A statistical trend (P=0.09) of lower percentage of SCE was observed in the CTRL group compared to O3SS (15.4 and 46.2 %, respectively). In conclusion, transcervical O3SS does not increase the number of endometrial PMNN and preventive treatment with O3SS applied to NHM cows did not decrease SCE. The results suggest a possible anti-inflammatory effect of the ionized saline solution treatment.
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