New Colorimetric Assay of Apurinic/Apyrimidinic (Abasic) Sites of Deoxyribonucleic Acid (DNA) Nucleotides Using Bicinchoninic Assay: A Bioanalytical Chemistry Experiment for the Undergraduate Curriculum
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Isaiah O. Stephens [1] ; Elizabeth M. Thomas [1]
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[1] Morehead State University
Morehead State University
Estados Unidos
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- Localización: Journal of chemical education, ISSN 0021-9584, Vol. 99, Nº 3, 2022, págs. 1454-1459
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Apurinic/apyrimidinic (AP or abasic) sites are deoxyribonucleic acid (DNA) lesions that result from the loss of a nucleobase by hydrolysis of the N-glycosyl bond. AP sites are the most frequent lesions in cells and are caused by environmental and cancer therapeutic genotoxins. Herein, the bicinchoninic colorimetric assay, typically used to quantify proteins, is used to quantify abasic sites within DNA nucleotides. The abasic sites can be quantified using either a traditional colorimeter to measure absorbances at 562 nm or using hand-held cameras to measure the color change (e.g., green value). Having a rapid colorimetric method for quantifying abasic sites within DNA nucleotides would be beneficial within undergraduate teaching laboratories to introduce students to DNA structures and how DNA lesions are produced structurally.
