Determination of genes involved in heat resistance response of Cronobacter sakazakii
- Autores: Juan Pablo Huertas Baquero, M. Ros, Mario Esteban Aranguez, Alfredo Palop Gómez, Colin Hill
- Localización: Actas del III Workshop en Investigación Agroalimentaria. WIA. 3.1 / Francisco Artés Hernández (dir. congr.), Marcos Egea Gutíerrez-Cortines (dir. congr.), Alfredo Palop Gómez (dir. congr.), Sebastián Bañón Arias (dir. congr.), Pablo Bielza Lino (dir. congr.), 2014, ISBN 978-84-697-1358-7, págs. 22-25
- Idioma: inglés
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Resumen
Cronobacter sakazakii is considered an emerging opportunistic pathogen causing meningitis, septicaemia and enterocilitis in neonates, related to the use of contaminated Powdered Infant Formula (PIF). C. sakazakii has an unusual surviving ability under dry conditions and has been suggested to be one of the most thermotolerant members of the Enterobacteriaceae. It is necessary to understand the molecular mechanisms underlying the thermal resistance of C. sakazakii and may ultimately be useful in the development of control strategies in PIF factories. In the current study, a transposon mutagenesis approach was used to identify the genes involved in heat resistance. A total of 23 mutants were found corresponding to 12 different defective genes. Heat resistance of selected mutants were determined with the use of the thermoresistometer Mastia. Only 2 mutants had a greater sensitivity to heat compared with the heat resistance of the wild type. Genes identified to be involved in the ...
