The purpose of this study was to validate whether the Gauntlet test (GT) can accurately estimate individual aerobic endurance performance compared with standard laboratory-based physiological tests. The GT required athletes to complete 5 maximal effort running stages, with a 1-minute break between each stage, with the goal of achieving the best overall time. Eighteen men (n = 9) and women (n = 9) (age, 23.5 +/- 4.13 years; body mass index, 23.1 +/- 7.62 kg[middle dot]m-2; 5k time, 22 +/- 7 minutes; 10k time, 47 +/- 15 minutes; V[Combining Dot Above]O2max, 52.3 +/- 8 ml[middle dot]kg-1[middle dot]min-1) completed a lactate threshold test and V[Combining Dot Above]O2max test (laboratory measures). Four to 14 days later, subjects completed the GT on an outdoor track. Blood lactate (bLa), V[Combining Dot Above]O2max, and heart rate (HR) were recorded during the laboratory session. Blood lactate, HR, stage completion time, and overall completion time were recorded during the GT. Linear regression correlation analyses revealed a significant inverse association between V[Combining Dot Above]O2max (mL[middle dot]kg-1[middle dot]min-1) and GT completion time (r = -0.88, P < 0.0001). In addition, there were significant correlations between V[Combining Dot Above]O2max maximum HR and GT maximum HR (r = 0.89, P < 0.0001) and V[Combining Dot Above]O2max 3-minute post bLa and GT 3-minute post bLa (r = 0.63, P = 0.0029). Sex-specific analysis showed significant inverse associations between female and male GT completion time and V[Combining Dot Above]O2max (r = -0.70, P = 0.0352; r = -0.94, P < 0.0002). Bland-Altman plots were used to evaluate concordance between GT completion time, V[Combining Dot Above]O2max, maximum HR, and 3-minute post bLa. Results suggest the GT is a valid assessment to accurately estimate aerobic endurance performance similar to standard laboratory methods.
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