Perfil químico, anti-5-lipoxigenase e efeitos inibitórios da ciclooxegenase do rizoma de gengibre (Zingiber officinale), do calo e calos tratados com elicitores

• Ammar Mohammed Ahmed Ali ^[3] ; Mawahib ElAmin Mohamed El-Nour ^[4] ; Abdulrahman Abdullah Al-Atar ^[5] ; Owais Mohammad ^[1] ; Mohamed Abdel-Raouf El-Sheikh ^[5] ; Ahmed Ali Qahtan ^[5] ; Eslam Mohamed Abdel-Salam ^[5] ; Sakina Mohamed Yagi ^[2]
  1. [1] Aligarh Muslim University

     Aligarh Muslim University

     India

     
  2. [2] University of Khartoum

     University of Khartoum

     Sudán

     
  3. [3] Department of Biology. Hajjah University. Yemen
  4. [4] Department of Biology and Biotechnology. Al Neelain University. Sudan
  5. [5] Department of Botany and Microbiology. University. Arábia Saudita

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• Localización: Ciencia rural, ISSN 0103-8478, Vol. 52, Nº. 10, 2022
• Idioma: portugués
• Títulos paralelos:
  • Chemical profile, anti 5-lipoxygenase and cyclooxegenase inhibitory effects of ginger (Zingiber officinale) rhizome, callus and callus treated with elicitors
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  • English

    The present study investigated the chemical profiles and evaluated the inhibitory effect against 5-Lipoxygenase (5-Lox) activity for extracts of ginger rhizome, callus, and callus treated with the elicitors; yeast extract (100, 300 and 500 mg/L), glycine (100, 200 and 300 mg/L) and salicylic acid (100 and 200 mg/L). Oils and chloroform: methanol (CM) extracts were prepared by maceration in petroleum ether and CM (1:1, v/v), respectively. Chemical profiles were determined by gas chromatography/mass spectrometry (GC/MS) analysis. Oil of the callus recorded higher 5-Lox inhibitory effect (IC50 58.33±4.66 μg/mL) than the oil of rhizome (IC50168.34±15.64 μg/mL) and comparable to that of the positive control; Nordihydroguaiaretic acid (IC50 61.25±1.02 μg/mL). The chemical profile of the callus oil contained large amounts of fatty acids, mainly the unsaturated fatty acid oleic acid (31.11%) and saturated fatty acid palmitic acid (28.56%). Elicitors modified the chemical profile of the callus and ameliorated the anti-5-Lox activity of CM extract of the callus. CM extracts of callus treated with 100 and 300 mg/L yeast extract and 50 mg/L salicylic acid significantly suppressed (P ≤ 0.05) the 5-Lox activity by 33.16%, 25.46% and 16%, respectively as compared to the CM extract of untreated callus. In conclusion, ginger callus could be considered as a valuable dietary supplement in the treatment of various inflammatory disorders

  • português

    The present study investigated the chemical profiles and evaluated the inhibitory effect against 5-Lipoxygenase (5-Lox) activity for extracts of ginger rhizome, callus, and callus treated with the elicitors; yeast extract (100, 300 and 500 mg/L), glycine (100, 200 and 300 mg/L) and salicylic acid (100 and 200 mg/L). Oils and chloroform: methanol (CM) extracts were prepared by maceration in petroleum ether and CM (1:1, v/v), respectively. Chemical profiles were determined by gas chromatography/mass spectrometry (GC/MS) analysis. Oil of the callus recorded higher 5-Lox inhibitory effect (IC50 58.33±4.66 μg/mL) than the oil of rhizome (IC50168.34±15.64 μg/mL) and comparable to that of the positive control; Nordihydroguaiaretic acid (IC50 61.25±1.02 μg/mL). The chemical profile of the callus oil contained large amounts of fatty acids, mainly the unsaturated fatty acid oleic acid (31.11%) and saturated fatty acid palmitic acid (28.56%). Elicitors modified the chemical profile of the callus and ameliorated the anti-5-Lox activity of CM extract of the callus. CM extracts of callus treated with 100 and 300 mg/L yeast extract and 50 mg/L salicylic acid significantly suppressed (P ≤ 0.05) the 5-Lox activity by 33.16%, 25.46% and 16%, respectively as compared to the CM extract of untreated callus. In conclusion, ginger callus could be considered as a valuable dietary supplement in the treatment of various inflammatory disorders