Importancia de la valoración de parámetros apoptóticos en muestras seminales para la selección de sementales

• A. Casao ^[1] ; A. Macias ^[2] ; A. Laviña ^[2] ; J.A. Cebrián Pérez ^[1] ; T. Muiño Blanco ^[1] ; R. Pérez Pé ^[1]
  1. [1] IUCA
  2. [2] ANGRA
• Localización: XVI Jornadas sobre Producción Animal: 19 y 20 de mayo de 2015, Zaragoza / Javier Álvarez Rodríguez (aut.), Begoña Panea Doblado (aut.), Jorge Hugo Calvo Lacosta (aut.), Mireia Blanco Alibés (aut.), José Alfonso Abecia Martínez (aut.), Daniel Villalba Mata (aut.), María Ángeles Latorre Górriz (aut.), Vol. 2, 2015, ISBN 978-84-606-7969-1, págs. 378-380
• Idioma: español
• Títulos paralelos:
  • Relevance of the evaluation of apoptotic parameters in ejaculates for apropiate selection of ovine males
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  • Texto Completo Libro
• Resumen

  • The aim of this study was to discover the reasons for the low fertility obtained after artificial insemination (<45%) with sperm samples from three rams (B3, B1 y B2) that had acceptable values of standard semen quality parameters (viability and motility). Comparisons of ejaculates of each animal with those of a male (A) who reached high values of fertility (66%) showed no significant differences in motility and viability (plasma membrane integrity) values between them. However, the study of apoptotic parameters revealed that male A had a significantly lower percentage of spermatozoa with inversion of phosphatidylserine (P <0.05) and DNA damage (P <0.05) when compared with males B3 and B2, but not with B1. No significant differences were observed in the percentage of spermatozoa with active caspases among low fertility and high fertility rams. Analysis of the capacitation state revealed that male A had a percentage of capacitated sperm significantly lower (P <0.05) than males with low fertility. On the basis of these preliminary results it would be advisable the inclusion of non-routine semen quality parameters in sire tests in order to improve the prediction of their fertilizing ability.