Resumen de La composición del diluyente influye en la dinámica de la fragmentación del adn espermático en el Morueco

J.L. Yániz, J. Gosálvez, C López-Fernández, P Santolaria

• This study was conducted to assess the effect of different semen extenders on the dynamics of sperm DNA fragmentation (SDF) in ram. Second ejaculates from 6 adult males were collected using an artificial vagina and diluted in either INRA-96®, milk, MOPS, TRIS, TES, HEPES, citrate or phosphate-based extenders. Semen samples were incubated at 37 ºC and the SDF were assessed after 0, 3, 6 and 24 h intervals. Significantly higher SDF indexes were obtained at 6 h and 24 h of incubation in the milk-based diluent than in all the buffer-based extenders. At 24 h, sperm diluted in INRA also reached a higher SDF than MOPS, TRIS, citrate, and phosphate-based extenders. The diluent had a clear impact on the pH of the samples, with MILK and INRA obtaining lower pH levels at the end of incubation period. It was concluded that the results of the dynamics of sperm DNA fragmentation are highly dependent on the diluent employed during incubation at 37 ºC, and this should be taken into account in studies evaluating this parameter.