Evaluación del coloide androcoll™ para seleccionar espermatozoides descongelados de ciervo

• I. Crespo Félez ^[1] ; S. Pardal ^[1] ; M. Mata Campuzano ^[1] ; A. Castañeda ^[1] ; J. Morrell ^[1] ; F. Martinez Pastor ^[1]
  1. [1] Universidad de León

     Universidad de León

     León, España

     
• Localización: XVI Jornadas sobre Producción Animal: 19 y 20 de mayo de 2015, Zaragoza / Javier Álvarez Rodríguez (aut.), Begoña Panea Doblado (aut.), Jorge Hugo Calvo Lacosta (aut.), Mireia Blanco Alibés (aut.), José Alfonso Abecia Martínez (aut.), Daniel Villalba Mata (aut.), María Ángeles Latorre Górriz (aut.), Vol. 2, 2015, ISBN 978-84-606-7969-1, págs. 370-372
• Idioma: español
• Títulos paralelos:
  • Evaluation of the androcoll™ colloid for thawed spermatozoa
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• Resumen

  • Selection of the optimal spermatozoa population is a necessary step in reproductive techniques, and it increases the quality and resilience of spermatozoa. Androcoll is a single-layer selection colloid that has been adapted to many species. We tested Androcoll-O-S (for sheep and small volumes) for selecting cryopreserved spermatozoa from red deer. Samples were collected from the cauda epididymis, frozen (20% egg yolk, 8% glycerol) and selected post-thawing. We tested two sample volumes, 150 and 300 μL, and two RCF, 300 and 600×g (20 min), in a 1-mL Androcoll volume. Analysis (flow cytometry, YO-PRO-1/propidium iodide/Mitotracker deep red) of selected cells (pellet) resulted in improved viability (87.3%±10.9), mitochondrial activity (86.5%±5.7) and apoptotic ratio (4.8%±2.2) than the unselected control (61.4%±10.8, 56.1%±8.2, 8.9%±2.7), with 300×g achieving slightly higher results for mitochondrial activity. Low quality spermatozoa (48.4%±9, 39.4%±10.2, 9.9%±5.1) were retained in the interphase. Recovery (selected/unselected cells, overall 29.1%±10.9) showed an interaction between volume and RCF, with increasing RCF not improving recovery when using 300 μL at 600×g (26.2%±10.1 vs. 34.3%±13.5 for 150 μL), maybe because a higher number of cells at these RCF could impair that high-quality cells enter the colloid. Androcoll-O-S is convenient and effective for selecting high-quality spermatozoa from thawed red deer.