Puesta a punto de marcadores mio y fibrogénicos relacionados con el desarrollo de la grasa intramuscular en vacuno de raza pirenaica

• L. Martinez Del Pino ^[1] ; O. Urrutia ^[1] ; A. Arana ^[1] ; L. Alonso ^[1] ; J.A. Mendizabal ^[1] ; B. Soret ^[1]
  1. [1] Universidad Pública de Navarra

     Universidad Pública de Navarra

     Pamplona, España

     
• Localización: XVI Jornadas sobre Producción Animal: 19 y 20 de mayo de 2015, Zaragoza / Javier Álvarez Rodríguez (aut.), Begoña Panea Doblado (aut.), Jorge Hugo Calvo Lacosta (aut.), Mireia Blanco Alibés (aut.), José Alfonso Abecia Martínez (aut.), Daniel Villalba Mata (aut.), María Ángeles Latorre Górriz (aut.), Vol. 2, 2015, ISBN 978-84-606-7969-1, págs. 456-458
• Idioma: español
• Títulos paralelos:
  • Mio and fibrogenic markers related to the development of intramuscular fat in pirenaica beef
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• Resumen

  • Fat, muscle, bone and connective tissue proceed from the same mesenchymal cells. A better knowledge of the relationship between myogenic and fibrogenic factors and intramuscular fat development could be of importance to improve quality of beef. RT-qPCR is an accurate technique to measure gene expression under different experimental conditions. Then, primers to analyze markers involved in myogenesis (Myf5, MyoD1, MyoG), fibrogenesis (FN1, FGFR1, FGF2, TGF`1) and myostatin (GDF8) were selected. Pirenaica young bulls (n=4) were used to obtain mRNA from the muscles Masseter, Longissimus dorsi and Semitendinosus. Efficiencies and R2 values were calculated, showing good adjustment. As the reliability of qPCR depends on normalization against stable reference genes, five genes were tested to find out the most suitable (B-actin, CNL3, RFLPO, PPIA and TOP2B).Based on the stability evaluation of the genes (RefFinder) TOP2B and B-actin were selected to be used as reference genes in this study. Finally, the preliminary results obtained with cDNA pools showed that Masseter presented lower expression of the myogenic markers and GDF8 while it had higher expression for some fibrogenic factors (FN1). These results could be related with the differences in metabolism and fiber composition of the muscles studied.