Resumen de Efecto de la alimentación con lino y algas sobre el desarrollo del tejido graso y la expresión de genes adipogénicos y lipogénicos de corderos
O. Urrutia, B. Soret, José Antonio Mendizábal Aizpuru, Antonio Purroy Unanua, A. Arana
This work aimed to study the effect of linseed (rich in 18:3n-3) oral gae (rich in EPA and DHA) supplementation on adipose tissue development and the expression of PPARG, CEBPA and SREBF1, FADS1, FADS2 and ELOVL5 in intramuscular (IM) and subcutaneous (SC) adipose tissues (AT). Thirty tree male Navarra breed lambs were assigned to three groups: control, C (barley and soya); L (C diet+10% linseed) and LA (C diet+5% linseed+3.7% algae). Although the contents of 18:1t11, 18:3n-3 and EPA in IM AT in L and LA groups increased (P < 0.001), there were not differences in IM fat content, which is consistent with no variations in adipocyte number and diameter, G3PDH activity or PPARG, CEBPA and SREBF1 expression. Conversely, the higher FA fluxes reaching SC tissue (stated by an increase in 18:1t11, 18:3n-3 and EPA) could enhance the lipogenesis process and triglyceride synthesis mediated by higher PPARG and CEBPA expression and higher G3PDH activity. mRNA levels of FADS1, FADS2 and ELOVL5 genes did not change in the SC tissue whereas FADS1 and FADS2 decreased in the IM tissue in L and LA. To conclude, IM and SC AT showed a tissue-specific response to n-3 PUFA supplementation.
