Detection of antibodies against Mycobacterium avium subsp.paratuberculosis in dairy goats by two ELISAs

• Autores: S. Rerkyusuke, M. Ganter
• Localización: Xl Congreso Nacional de la Sociedad Española de Ovinotecnia y Caprinotecnia (SEOC) XVL Congreso Internacional / coord. por María Jesús Alcalde Aldea, Martín Rodríguez García, 2015, ISBN 978-84-9048-398-5, págs. 469-475
• Idioma: inglés
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• Resumen

  • The objective was to evaluate two commercial ELISAs for detection ofantibody activities against Mycobacterium avium subsp. paratuberculosis(MAP) in goats. Therefore 3 groups of goat sera from one flock including283 goats vaccinated with Gudair® against paratuberculosis (group 1),39 goats with clinical paratuberculosis, confirmed by culture and PCRtechnique (group 2), and 58 motherless reared unvaccinated replacementkids (group 3) were tested with LDL-ELISA and IDEXX-ELISA. In thevaccinated group 1, MAP antibody activities were analysed each yearafter vaccination for three years by LDL-ELISA. In addition, the patternsof antibody activities over 3 years after vaccination were analysed.The sensitivity in group 1 was 84%, and 36% in LDL-ELISA and IDEXXELISA,respectively. Sensitivity in group 2 was 85% and 56% in LDLELISAand IDEXX-ELISA, respectively. The specificity in the MAPnegative group 3 was 100%. After vaccination, MAP antibody activitiesdeclined significantly the 3 consecutive years with both ELISAs (p<0.05).84.3% of healthy goats had high antibody activities but 11.8% of themhad negative or inconclusive results three years after vaccination withLDL-ELISA.After Gudair® vaccination a differentiation between MAP infected anduninfected goats is only possible the following two years.