Interferencia de las mucinas intestinales en la determinación de fibra dietética total de la digesta en conejos

• R. Abad Guamán ^[1] ; Carlos Romero ; R. Carabaño ^[1] ; J. Garcia ^[1]
  1. [1] European Telecommunications Standards Institute

     European Telecommunications Standards Institute

     Arrondissement de Grasse, Francia

     
• Localización: XV Jornadas sobre Producción Animal: 14 y 15 de mayo de 2013, Zaragoza / Jorge Hugo Calvo Lacosta (aut.), Isabel Casasús Pueyo (aut.), Margalida Joy Torrens (aut.), Javier Álvarez Rodríguez (aut.), Luis Varona Aguado (aut.), Begoña Panea Doblado (aut.), Carlos Calvete Margolles (aut.), Joaquim Barcells Teres (aut.), Vol. 1, 2013, ISBN 978-84-695-7684-7, págs. 198-200
• Idioma: español
• Títulos paralelos:
  • Interference of intestinal mucins in the determination of total dietary fibre of digesta in rabbits
• Enlaces
  • Texto Completo Libro  1 (pdf)   2 (pdf) 
• Resumen

  • The aim of this work was to study the potential interference between intestinal mucin and the determination rabbit digesta of total dietary fibre (TDF). Apparent crude mucin was determined in rabbit jejunal raw mucus (free of digesta), ileal digesta, faeces, and three ingredients free of crude mucin [sugar beet pulp pectin, diet containing 26% sugar beet pulp (SBP) and lignocellulose]. After crude mucin analysis the residue obtained from each sample was treated enzymatically with a pectinase to eliminate potential contamination of mucin with soluble fibre. Raw mucus was analyzed for TDF. Raw mucus and SBP pectin showed a similar content in crude mucin (739 and 709 g/kg DM, respectively), but when this residue was treated with pectinase the value for SBP pectin decreased up to 1.8 g/kg DM but remained almost unchanged for raw mucus (726 g/kg DM). Positive but much lower mucin values were also recorded for the diet and lignocelluloses, but they became close to zero after pectinase treatment. Faeces contained lower mucin concentration than ileal digesta (10.7 vs. 86.4 g/kg DM, respectively) and the pectinase barely modified faecal mucins but reduced those of ileal digesta (10.3 vs 74.6% g/kg DM) probably due to its higher soluble fibre content. Protein content of ileal and faecal mucins were 24.3 and 21.7% DM, respectively. Raw mucus contained 571 gTDF/kg DM. The mucin content retained in ileal/faecal TDF might be estimated as: ileal/faecal mucin concentration divided by 0.739 (to obtain the total raw mucus in ileal/faecal digesta), discounted its protein content (as TDF is expressed free of protein), and then multiplying this value by the proportion of mucins retained in TDF analysis (x 0.571).