Targeting AURKA by microRNA-490-3p suppresses gastric cancer cell growth

• Rui Chen ^[1] ; Shenkang Zhou ^[1] ; Chengfeng Fang ^[1] ; Feifei Ye ^[1] ; Jianhui Chen ^[1] ; Pinlu Jiang ^[1]
  1. [1] Taizhou Hospital of Zhejiang Province, Taizhou, Zhejiang, PR China
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 37, Nº. 4, 2022, págs. 397-404
• Idioma: inglés
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• Resumen

  • Objective. To illustrate the molecular mechanism of microRNA-490-3p regulating gastric cancer (GC) cells by targeting AURKA.

    Methods. Genes with significantly different expression in GC and normal tissue in TCGA-STAD dataset were analyzed by bioinformatics. Expression levels of genes and proteins in GC cells were measured by qRT-PCR and western blot. The interaction between microRNA-490-3p and AURKA was verified by dual luciferase assay. Proliferation, migration, invasion and apoptosis of GC cells were evaluated through a set of cell function assays.

    Results. MicroRNA-490-3p was significantly less expressed in GC, while AURKA was significantly highly expressed. Dual luciferase reporter gene assay proved that microRNA-490-3p targeted AURKA. Upregulation of microRNA-490-3p restrained proliferation, migration, invasion and stimulated apoptosis of GC cells, which was attenuated by overexpression of AURKA.

    Conclusions. MicroRNA-490-3p was likely to restrain the development of GC cells by inhibiting AURKA, and it may be an underlying target for GC treatment.