Resumen de High-level aminoglycoside resistance enterococcus spp in a tertiary care hospital in mexico

José Arellano, Yazmín Garzón Tejada, Silvia Giono Cerezo, Olga Mateos Salazar, Efrén Alberto Pichardo Reyes

• español

  Enterococcus es una causa importante de infección intrahospitalaria. Se determinaron los niveles altos de resistencia para aminoglucósidos(HLAR), gentamicina (HLGR) y estreptomicina (HLSR) en Enterococcus aislados de diversos casos clínicos en un hospital de tercer nivel en México, D.F.

  La identificación se realizó usando el sistema de Microscan® y la resistencia a ampicilina, estreptomicina, gentamicina, vancomicina, HLGR y HLSR de acuerdo a la NCCLS. 91 cepas fueron aisladas de muestras clínicas de Enero de 1998 a Enero 1999, se identificaron dos especies. 83 (91.2%) E. faecalis y 8/91 (8.8%) fueron E. faecium. 67/91 (73.6%) E. faecalis se aislaron de muestras de orina. Ninguna cepa mostró resistencia a vancomicina; 1/8 E. faecium fue ampicilina resistente. 30/83 (36%) E. faecalis y 3/8 E. faecium fueron resistentes a gentamicina, mientras que 39/83 (47.0%) E. faecalis y 4/8(50%) E. faecium fueron resistentes a estreptomicina. Además 14/83(16%) E. faecalis y 3/8 E. faecium fueron sensibles para ambos aminoglucósidos (gentamicina y estreptomicina). Ninguna cepa fué productora de -lactamasas. 12/83 (14.4%) E. faecalis mostraron HLGR y 28/83 (33.7%) HLSR. 2/8 E. faecium fueron HLGR y 2/8 fueron HLSR. De los aislamientos de orina 33/83 (39.7%) E. faecalis fueron HLAR y 3/8(37.5%) E. faecium. E. faecalis fue más frecuente que E. faecium. Este trabajo muestra que Enterococcus ssp. HLAR se aisla y podría ser un problema en brotes de infección nosocomial. .

• Multiple

  Enterococcus is one important cause hospital-acquired infections. High levels of resistance for aminoglycosides (HLAR) as gentamicin (HLGR) and streptomycin (HLSR) in Enterococcus isolates in a tertiary clinical care in Mexico City were studied.

  Identified using Microscan® system. Resistance to ampicillin, streptomycin, gentamicin and vancomycin according to NCCLS. HLGR and HLSR were confirmed using disks. 91 strains were isolated and identified from clinical samples from January 1998 to January 1999. Two species were identified. 83 (91.2 %) E. faecalis and 8/91 (8.8 %) were E. faecium. E. faecalis in urine samples were 67/91 (73.6%). Neither showed vancomycin or ampicillin resistance; 1/8 E. faecium was ampicillin resistant. 30/83 (36%) E. faecalis and 3/8 E. faecium were gentamicin resistant; while 39/83 (47.0%) E. faecalis and 4/8(50%) E. faecium were streptomycin resistant. 14/83 (16%) E. faecalis, 3/8 E. faecium showed sensitive pattern for gentamicin and streptomycin. None strains were -lactamases producer. E. faecalis 12/83 (14.4%) were HLGR and 28/83 (33.7%) were HLSR. E.

  faecium. 2/8 were HLGR and 2/8 were HLSR. HLAR 33/83 (39.7%) were E. faecalis and 3/8(37.5%) were E. faecium isolated from urine. E. faecalis was more frequent than E. faecium and show that HLAR in Enterococci is high and could be a serious problem if spread as nosocomial infection