Circular RNA hsa_circ_0003892 promotes the development of papillary thyroid carcinoma by regulating the miR-326/LASP1 axis

• Peng Han ^[1] ; Junsong Liu ^[1] ; Qian Zhao ^[1] ; Honghui Li ^[1] ; Ting Zhang ^[1] ; Baiya Li ^[1] ; Xiaorong Niu ^[1]
  1. [1] First Affiliated Hospital of Xi'an Jiaotong University

     First Affiliated Hospital of Xi'an Jiaotong University

     China

     
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 38, Nº. 5, 2023, págs. 585-595
• Idioma: inglés
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• Resumen

  • Background. Thyroid cancer is the most common malignancy of the endocrine system. Circular RNA (circRNA) is recognized as a key regulator of tumorigenesis in papillary thyroid carcinoma (PTC).

    Here this work focused on the mechanism of circRNA_0003892 (circ_0003892) in PTC progression.

    Methods. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to examine circ_0003892, microRNA-326 (miR-326) and LIM and SH3 protein 1 (LASP1) mRNA expression levels in PTC tissues and cell lines. Besides, cell counting kit-8 (CCK-8), EdU and transwell assays were conducted to detect the proliferative, migrative and invasive abilities of PTC cells, respectively. B The targeting relationships between miR-326 and circ_0003892 or LASP1 3'-UTR were verified by dual-luciferase reporter gene assay and RNA immunoprecipitation (RIP) assay.

    Results. Circ_0003892 expression was raised in PTC tissues and cells, which was significantly interrelated with larger tumor size and extrathyroidal extension in PTC sufferers. Overexpression of circ_0003892 significantly promoted the malignant biological behaviors of PTC cells. Additionally, miR-326 was a downstream target of circ_0003892, and miR-326 overexpression weakened the promoting effect of circ_0003892 overexpression on the malignant progression of PTC. MiR-326 specifically inhibited LASP1. Circ_0003892 positively regulated LASP1 expression by targeting miR-326.

    Conclusion. Circ_0003892 up-regulates LASP1 expression and facilitates PTC progression via competitively binding to miR-326.