Host-Associated Bacterial Communities Vary Between "Daphnia galeata" Genotypes but Not by Host Genetic Distance

• Amruta Rajarajan ^[1] ; Justyna Wolinska ^{[2] [4]} ; Jean-Claude Walser ^[3] ; Stuart R. Dennis ^[1] ; Piet Spaak ^[1]
  1. [1] Swiss Federal Institute of Aquatic Science and Technology

     Swiss Federal Institute of Aquatic Science and Technology

     Dübendorf, Suiza

     
  2. [2] Leibniz Institute of Freshwater Ecology and Inland Fisheries

     Leibniz Institute of Freshwater Ecology and Inland Fisheries

     Berlin, Stadt, Alemania

     
  3. [3] Swiss Federal Institute of Technology in Zurich

     Swiss Federal Institute of Technology in Zurich

     Zürich, Suiza

     
  4. [4] Department of Biology, Chemistry, Pharmacy, Institut Für Biologie, Freie Universität Berlin (FU), Berlin, Germany

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• Localización: Microbial ecology, ISSN-e 1432-184X, ISSN 0095-3628, Vol. 85, Nº. 4, 2023, págs. 1578-1589
• Idioma: inglés
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  • Host genotype may shape host-associated bacterial communities (commonly referred to as microbiomes). We sought to determine (a) whether bacterial communities vary among host genotypes in the water flea Daphnia galeata and (b) if this difference is driven by the genetic distance between host genotypes, by using D. galeata genotypes hatched from sediments of different time periods. We used 16S amplicon sequencing to profile the gut and body bacterial communities of eight D. galeata genotypes hatched from resting eggs; these were isolated from two distinct sediment layers (dating to 1989 and 2009) of a single sediment core of the lake Greifensee, and maintained in a common garden in laboratory cultures for 5 years. In general, bacterial community composition varied in both the Daphnia guts and bodies; but not between genotypes from different sediment layers. Specifically, genetic distances between host genotypes did not correlate with beta diversity of bacterial communities in Daphnia guts and bodies. Our results indicate that Daphnia bacterial community structure is to some extent determined by a host genetic component, but that genetic distances between hosts do not correlate with diverging bacterial communities.