MiR-195-5p suppresses gastric adenocarcinoma cell progression via targeting OTX1

• Sizhe Hu ^[1] ; Huanting Zhou ^[1] ; Xiaokang Zhao ^[1] ; Feng Qian ^[1] ; Cancan Jin ^[1]
  1. [1] Affiliated Dongyang People’s Hospital of Wenzhou Medical University, Dongyang, Zhejiang, China
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 38, Nº. 6, 2023, págs. 659-668
• Idioma: inglés
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• Resumen

  • Gastric adenocarcinoma (GAC) caused by malignant transformation of gastric adenocytes is a malignancy with high incidence. MiR-195-5p modulates a variety of cancers. One of its target genes, orthodenticle homeobox 1 (OTX1), is believed to be a key modulator of tumor progression. We aim to analyze the mechanism of miR-195-5p and OTX1 in GAC. MiR195-5p and OTX1 mRNA levels in GAC cells were tested via qRT-PCR. OTX1 protein and EMT-related protein levels were examined through western blot.

    Several cell functional assays were designed to measure changes in cell malignant behaviors. Dual luciferase assay verified the targeting relation of miR-195-5p and OTX1. These experimental results showed significantly low miR-195-5p expression and significantly high OTX1 expression in GAC cells. Enforced miR-195-5p level repressed cell malignant progression and accelerated cell apoptosis in GAC. Increased OTX1 weakened the above-mentioned effect caused by overexpressing miR-195-5p. Thus, miR-195-5p restrained migration, proliferation, invasion and epithelial-mesenchymal transition process of GAC cells, and promoted cell apoptosis through regulating OTX1.

    A new insight is provided for searching for biomarkers or therapeutic targets of GAC.