Fourier transform infrared spectrometer analysis and antimicrobial screening of ethanolic extract of Operculina terpathum from cholistan desert

• Ghazala Shaheen ^[1] ; Arslan Ashfaq ^[3] ; Anum Khawar ^[4] ; Qurratul Ain Jamil ^[1] ; Rashida Parveen ^[2] ; Faheem Hadi ^[1] ; Aymen Owais Ghauri ^[5] ; Jafir Hussain Shirazi ^[1] ; Hafiz Muhammad Asif ^[1] ; Tahira Shamim ^[1] ; Laila Sumreen ^[1] ; Tanveer Ali ^[3] ; Muhammad Akram ^[6] ; Raessa Noor ^[1] ; Amna Mehmood ^[3] ; Farhan Sajid ^[1]
  1. [1] Islamia University of Bahawalpur

     Islamia University of Bahawalpur

     Pakistán

     
  2. [2] Superior University

     Superior University

     Pakistán

     
  3. [3] Faculty of Eastern Medicine and Natural Sciences, Ziauddin University Karachi, Pakistan.
  4. [4] Medical officer, Rural Health Center, RHC Chung Lahore. Pakistan
  5. [5] Department of Eastern Medicine, Faculty of Allied Health Sciences, Jinnah University for Women, Karachi, Pakistan.
  6. [6] Department of Eastern Medicine, Government College University Faisalabad, Pakistan.

  Mostrar afiliaciones +
• Localización: Pharmacy Practice (Granada), ISSN-e 1886-3655, Vol. 20, Nº. 2, 2022 (Ejemplar dedicado a: Apr-Jun)
• Idioma: inglés
• Enlaces
  • Texto completo (pdf)
• Resumen

  • Aim of present study was to assess pharmacological (antioxidant, antibacterial & antifungal) potential of Operculina terpathum seeds. Ethanolic extract was prepared and its phytochemical evaluation show the different chemical compounds such as carbohydrates, phenols, tannin, flavonoids, cardiac glycosides, steroids, alkaloids and proteins. FTIR spectra showed the presence of organic acids, hydroxyl and phenolic compounds, amino groups, aliphatic compounds, functional groups such as amide, ketone, aldehyde, aromatics and halogen compounds. Antioxidant activity of the Operculina terpathum alcoholic extract was performed by DPPH method and it showed 97.13%whereas IC50±SEM (μg/ml) was 1.425±0.16. Antibacterial activity was performed against different bacterial strains and results were comparable with that of standard. Maximum antibacterial activity was exhibited by Bacillus subtillis (28.33±2 mm) and Bacillus pumilus (25.33±2 mm) respectively. Antifungal activity was also performed and it showed maximum activity against Aspergillus flavous and Candida albicans6±1, 5±1mm respectively. These results showed that Operculina terpathum has good antibacterial and antifungal activity against different microbes and it could be used as an alternative to antibiotics, as the antibiotics resistance is very common now a days