Hyperin up-regulates miR-7031-5P to promote osteogenic differentiation of MC3T3-E1 cells

• Dongchen Qian ^[1] ; Yueyue Chen ^[1] ; Xusheng Qiu ^[1] ; Baohua Zhu ^[1] ; Lin Zhang ^[1] ; Yifeng Yan ^[1] ; Yixin Chen ^[1]
  1. [1] Hospital of Nanjing University Medical School, Nanjing, PR China
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 38, Nº. 10, 2023, págs. 1219-1229
• Idioma: inglés
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• Resumen

  • Objective. To investigate the effects of Hyperin (Hyp) on osteogenic differentiation of MC3T3- E1 cells.

    Methods. Differentially expressed miRNA was screened by miRNA Microarray. miR-7031-5P overexpression and knockdown MC3T3-E1 cell models were constructed by transfecting miR-7031-5P mimics and inhibitor. Alizarin red staining (ARS) assay was used to observe the formation of mineralized nodules in MC3T3-E1 cells. ALP activity was detected by using ALP detection kit. Western blot assay was used to examine the changes in osteogenic differentiation-related proteins. The relationship between miR-7031-5P and Wnt7a was revealed by dual luciferase report experiments.

    Results. We found that miR-7031-5P was upregulated in MC3T3-E1 cells after Hyp treatment. The results indicated that compared with the untreated group, Hyp promoted the formation of mineralized nodules and the alkaline phosphatase (ALP) activity of MC3T3-E1 cells via overexpressing miR-7031-5P. Besides, elevated miR-7031-5P increased OPN, COL1A1, and Runx2 mRNA expression. More importantly, Wnt7a was identified as the downstream target gene of miR-7031- 5P promoting osteogenic differentiation of MC3T3-E1 cells.

    Conclusions. Hyp up-regulated miR-7031-5P to promote osteogenic differentiation of MC3T3-E1 cells by targeting Wnt7a.