Glioblastoma research on zebrafsh xenograft models: a systematic review

• Alexandra Pliakopanou ^[1] ; Ilias Antonopoulos ^[1] ; Nikolia Darzenta ^[1] ; Iliana Serifi ^[2] ; Yannis Vasilios Simos ^[1] ; Andreas Panagiotis Katsenos ^[1] ; Stefanos Bellos ^[1] ; George Athanasios Alexiou ^[3] ; Athanasios Petros Kyritsis ^[3] ; Ioannis Leonardos ^[4] ; Patra Vezyraki ^[1] ; Dimitrios Peschos ^[1] ; Konstantinos Ioannis Tsamis ^[1]
  1. [1] Laboratory of Physiology, Faculty of Medicine, School of Health Sciences, University of Ioannina, Greece
  2. [2] Laboratory of Biological Chemistry, Faculty of Medicine, School of Health Sciences, University of Ioannina, Greece
  3. [3] Neurosurgical Institute, University of Ioannina, Greece
  4. [4] Zoology Laboratory, Department of Biological Application and Technology, University of Ioannina, Greece

  Mostrar afiliaciones +
• Localización: Clinical & translational oncology, ISSN 1699-048X, Vol. 26, Nº. 2, 2024, págs. 311-325
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Glioblastoma (GBM) constitutes the most common primary brain tumor in adults. The challenges in GBM therapeutics have shed light on zebrafish used as a promising animal model for preclinical GBM xenograft studies without a standardized methodology. This systematic review aims to summarize the advances in zebrafish GBM xenografting, compare research protocols to pinpoint advantages and underlying limitations, and designate the predominant xenografting parameters. Based on the PRISMA checklist, we systematically searched PubMed, Scopus, and ZFIN using the keywords “glioblastoma,” “xenotransplantation,” and “zebrafish” for papers published from 2005 to 2022, available in English. 46 articles meeting the review criteria were examined for the zebrafish strain, cancer cell line, cell labeling technique, injected cell number, time and site of injection, and maintenance temperature. Our review designated that AB wild-type zebrafish, Casper transparent mutants, transgenic Tg(fli1:EGFP), or crossbreeding of these predominate among the zebrafish strains. Orthotopic transplantation is more commonly employed. A number of 50–100 cells injected at 48 h post-fertilization in high density and low infusion volume is considered as an effective xenografting approach. U87 cells are used for GBM angiogenesis studies, U251 for GBM proliferation studies, and patient-derived xenograft (PDX) to achieve clinical relevance. Gradual acclimatization to 32–33 °C can partly address the temperature differential between the zebrafish and the GBM cells. Zebrafish xenograft models constitute valuable tools for preclinical studies with clinical relevance regarding PDX. The GBM xenografting research requires modification based on the objective of each research team. Automation and further optimization of the protocol parameters could scale up the anticancer drug trials.