Effects of seed and vegetative stage cysteine treatments on oxidative stress response molecules and enzymes in Ocimum basilicum L. under cobalt stress
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[1] Shahid Bahonar University Faculty of Science Department of Biology
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- Localización: Journal of soil science and plant nutrition, ISSN-e 0718-9516, ISSN 0718-9508, Vol. 15, Nº. 3, 2015, págs. 651-662
- Idioma: inglés
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Resumen
In this research, the effects of seed and vegetative stage cysteine treatments on the oxidative stress response of molecules and enzymes in Ocimum basilicum L. under cobalt stress were studied. Parameters such as proline content, lipid peroxidation, leaf protein content, ascorbate peroxidase, catalase, phenylalanine ammonia-lyase and polyphenoloxidase activity were measured. Research was conducted in two separate trials including seed treatment and the treatment of plant at vegetative stage. In both cases, cobalt treatment was done in vegetative stage. Concentrations of 0, 0.5 and 2.5 mM cysteine and 0, 100 and 500 µM cobalt were used. Higher concentrations of cobalt increased MDA, other aldehydes, proline content and activity of CAT, APX, and PPO compared with the control (without cobalt and cysteine). Cobalt stress decreased leaf protein content compared with the control. Seed treatment with cysteine reduced the MDA, other aldehydes, proline, leaf protein content and APX activity of plants compared to plant treated with cobalt and without cysteine. Plant treatment with cysteine at vegetative stage caused an increase in leaf protein and a decrease in MDA, other aldehydes, proline, content and activity of CAT, APX and PPO. The results showed that seed treatment with cysteine could alleviate cobalt stress effects in lower concentrations while cysteine applied invegetative stage was more effective at higher concentrations of cobalt. Based on these results probably cysteine due to protection of membrane decreased oxidative stress created by cobalt treatment.
