Assessment of bioavailability of trace elements, such as zinc (Zn), and lead (Pb), in polluted soils is needful to management and use of these soils. A greenhouse experiment was performed to investigate bioavailability of Zn and Pb for bean by various extractants (DTPA-TEA, AB-DTPA, Mehlich 2, Mehlich 3, 0.01 M CaCl2, and 0.1 M HCl) in 10 polluted soils. The results showed that extraction capacity was as follows: Mehlich 3>AB-DTPA>DTPA-TEA>Mehlich 2>CaCl2>HCl. The average of Zn extracted by Mehlich 3, AB-DTPA, and DTPA-TEA were 25.68, 9.73, and 7.30 mg kg-1, respectively. In addition, the average of Zn extracted by Mehlich 2, CaCl2, and HCl were 6.53, 0.13, and 0.11 mg kg-1, respectively. The average of Pb extracted by Mehlich 3, AB-DTPA, and DTPA-TEA were 12.56, 7.32, and 4.91 mg kg-1, respectively. Also, the average of Pb extracted using Mehlich 2, CaCl2, and HCl were 0.93, 0.62, and 0.44 mg kg-1, respectively. The results of correlation analysis revealed that the Zn extracted by DTPA-TEA, AB-DTPA, Mehlich 3, Mehlich 2, and HCl correlated significantly with Zn uptake. Moreover, significant correlation was found between DTPA-TEA, AB-DTPA, HCl, and CaCl2 with Zn concentration in bean. The concentration of Pb in bean and Pb extracted by DTPA-TEA, AB- DTPA, Mehlich 3, and Mehlich 2 correlated significantly. Therefore, application of extracting methods contains chelating agents would be recommended in the future research on the availability of Zn and Pb in polluted soils.
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