Comparación de métodos para la recuperación y determinación de la prevalencia de Streptococcus agalactiae en mujeres gestantes de Medellín

• Autores: Clara M. Duque, Beatriz Gómez, Olga l. Uribe, Marcela Gutiérrez, Erika Ruiz, Gladis A. Leudo, Sandra S. Montiel
• Localización: Infectio: Revista de la Asociación Colombiana de Infectología, ISSN-e 2422-3794, ISSN 0123-9392, Vol. 14, Nº. 2, 2010
• Idioma: español
• Títulos paralelos:
  • Method Comparison for the Recovery from Streptococcus agalactiae and Its Prevalence Determination in Pregnant Women of Medellín
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• Resumen
  • español

    Streptococcus agalactiae es un coco Gram positivoque se encuentra frecuentemente asociadoa infecciones en neonatos, mujeres embarazadasy adultos inmunocomprometidos.

  • English

    Objective: Compare method sensitivity for recovery from S. agalactiae and to determine its prevalence in a group of pregnant women of the city of Medellín.Materials and methods: Cross-sectional descriptive study. The population was pregnant women of Medellín who consulted from February to October, 2008; 362 pregnant women were included; the samples were taken from vagina introitus and anal area. The samples were simultaneously cultured in New Granada Agar and Todd Hewitt Broth supplemented with antibiotics, which was the base for the performance of a subculture in Ram Blood Agar at 5%.Results: When comparing the New Granada Agar method of recovery with the Todd Hewitt Broth gold test, the sensitivity of the first was 44% and the positive predictive value was 58%; it presented 98% specificity and a negative predictive value of 99%. S. agalactiae was found in 21 (5.8%)pregnant women.Conclusions: Todd Hewitt Broth is a sensitive method for the recovery from S. agalactiae; therefore, continuing with this methodology for the screening of pregnant women in our environment is recommended. The 5,8%  prevalence found in this study is consistent with the reports in world-wide literature. Considering that the rate of vertical transmission is approximately 50%, the need to implement epidemiological surveillance measures in ourenvironment is evident.