Bacterias patógenas con alta resistencia a antibióticos: estudio sobre reservorios bacterianos en animales cautivos en el zoológico de Barranquilla

• Autores: Julio Vargas, Salim Mattar, Santiago Monsalve
• Localización: Infectio: Revista de la Asociación Colombiana de Infectología, ISSN-e 2422-3794, ISSN 0123-9392, Vol. 14, Nº. 1, 2010
• Idioma: español
• Títulos paralelos:
  • Captive animals at Barranquilla’s zoo are reservoirs of high resistance bacterial pathogens
• Enlaces
  • Texto completo
• Resumen
  • español

    Materiales y métodos: Se tomaron muestrasde cloacas y glotis de 30 aves, y frotisrectales y nasales de 29 mamíferos, los animalesfueron inmovilizados mecánicamentey, luego, anestesiados. Las bacterias se identificaroncon pruebas bioquímicas como:urea, SIM (sulfide-indole-motility medium),TSI (triple sugar iron), LIA (line immunoassay)y citrato. Algunos aislamientos se confirmaroncon el sistema API 20E (Biomerieux, S.A.,Marcy I’Etoile, France) o Microscan® Negcombo panel type 32 (Dade behring, CA,USA). La sensibilidad a los antimicrobianosse evaluó con el método de Bauer y Kirby,teniendo en cuenta las normas del Clinicaland Laboratory Standards Institute (CLSI).

  • English

    Objective: The objective is to determine respiratory and enteric bacterial pathogens and antimicrobial susceptibility in captive animals at Barranquilla Zoo.Materials and methods: Samples were taken from rectus and glottis in 30 birds and nasal and rectal swabs from 29 mammals, which were restricted mechanically and then anesthetized. Bacteria were identified by usingbiochemical tests such as: Urea, SIM, TSI, LIA and Citrate, some bacteria isolates were confirmed with API 20E (Biomerieux SA, Marcy I’Etoile, France) or Micro scan® Neg combo panel type 32 (Dade Behring, CA, USA).  Antimicrobial susceptibility was assessed using the Bauer and Kirby method and taking into account CLSI regulation. Results Eighty-nine strains were obtained, 45 from birds and 44 from mammals. The most frequent bacteria were: E. coli (n = 31), Klebsiella pneumoniae (n = 20), Enterobacter cloacae (n = 10), Pseudomonas aeruginosa (n = 5), Staphylococcusaureus (n = 5) and Pseudomonas stuzeri (n = 4). Gram negative bacteria and  Staphylococci were respectively resistant to the following antibiotics: tetracycline (28% and 57.1%), chloramphenicol (14.6% and 57.1%) and ?-lactam (54.2% and 42.8); Gram-negative was (6.1%) resistant to Fluorquinolones and (2.4%) resistant to aminoglycosides; and Staphylococciwere (64.2%) resistant to macrolides. Twentyfour (27%) were multi- resistant: 16 (36%) in birds and 8 (18%) in mammals.Conclusion: Resistance to one or more antibiotics in bacteria isolated fromcaptive animals Barranquilla Zoo, is a risk factor for health for the animalsthemselves. The zoo animals are potential reservoirs for bacteria and  resistance genes clinically important in the spread of these resistance factors. The resistance similarities found in animal and human strains  suggest clone mobility between the sapiens species and the animals.