Resumen de Aislamiento e identificación de Enterobacter sakazakii en lactarios de Bogotá, D. C.

María Consuelo Vanegas, Laura Cristina Rugeles, Aída Juliana Martínez

• español

  Introducción. Enterobacter sakazakii es unpatógeno oportunista emergente de alto riesgo,responsable de meningitis grave y enterocolitisnecrosante. El principal vehículo detransmisión de esta bacteria son los productosinfantiles deshidratados, debido a su contaminacióndespués del tratamiento térmico.Objetivo. Identificar cepas de E. sakazakiien muestras de lactarios recolectadas en laciudad de Bogotá, D.C.Materiales y métodos. Se analizaron 222muestras de 9 lactarios, de superficies estérilesy no estériles, utensilios empleados para la preparaciónde biberones y operarios. Se realizórecuento de coliformes totales y detección deE. sakazakii utilizando el protocolo propuestopor la Food and Drug Administration y por elSistema Automático Bax® Dupont Qualicon.

• English

  Introduction: Enterobacter sakazakii is an emergent opportunistic pathogen of high risk responsible of severe meningitis and necrotizing enterocolitis in neonates and infants with underlying medical conditions. One of the principal transmission vehicles for the transmission of these bacteria, is the infant dehydrated formula after exposing them to the heating treatment.Objective: To identify strains of E. sakazakii in milk feeders’ samples from Bogotá. Materials and methods: 222 samples from 9 milk feeders including sterile and non sterile surfaces, utensils used for the formula preparation and food handlers were analyzed. Total coliforms counts and identification of E. sakazakii was done using the FDA protocol and the automatic system Bax ® Dupont Qualicon.Results: From de 222 samples collected from hospitals in Bogotá, it was reported that 27.4% (61) had total coliforms, and the presence of E. sakazakii was detected in 3.6% (8) from one feeding bottle and surfaces.Conclusion: The presence of E. sakazakii strains was reported in Colombian milk feeders. Because this microorganism is a high risk opportunistic pathogen for newborn infants, usually associated with hygiene practices inmilk feeders, the information of this research could be useful to develop preventive measurements to reduce the risk of contamination in the infant population and provides important public health information.