Resumen de Caracterización molecular de los genes histona H2A y ARNsno-Cl de Trypanosoma rangeli: aplicación en pruebas diagnósticas
Paula Ximena Pavía, Claudia L. Cuervo, Juliana Gil, Ibeth Romero, Liliana Morales, Hugo Díez, Claudia Quintero, Patricia del Portillo, Gustavo Adolfo Vallejo, Astrid C. Florez, Marleny Montilla Moreno, Concepción Judith Puerta Bula
- español
La aplicación de la reacción en cadena de lapolimerasa (PCR) para detectar e identificarTrypanosoma cruzi y Trypanosoma rangeli presentaa menudo dificultades de interpretación.Así, algunas pruebas generan la amplificaciónde bandas similares provenientes de uno delos dos parásitos, fragmentos polimórficos deun mismo parásito, o la prevalencia en la detecciónde T. cruzi en infecciones mixtas.
- English
The application of polymerase chain reaction (PCR) to detect Trypanosoma cruzi and Trypanosoma rangeli often presents interpretation challenges. For example, some tests yield the amplification of similar bands from either parasite, polymorphic fragments of the same parasite, or present deviation towards T. cruzi in mixed infections.In this study, the basic researching needed for designing and standardizating specific PCR tests for each parasite species PCR are shown and analyzed. The TcH2AF/R primers were designed on the basis of the differential gene region observed between the histone h2a genic units of these parasites. These primers amplify a specific 234 bp fragment in T. cruzi (T. cruzi I and II strains). The TrF/R2 primers anneal to the intergenic regions of an 801 bp gene fragment encoding for six transcripts that conform the snoRNA-Cl cluster in T. rangeli. These primers amplify a fragment of 620 bp exclusively in KP1(-) and KP1(+) strains of the parasite.The application of these PCR tests in infected vectors and in chagasic patients show that both tests constitute useful tools for the diagnosis and differential identification of these trypanosomatids.
