Resumen de Aislamiento de Staphylococcus epidermidis portador de integrón clase 1 en un paciente con sepsis neonatal

Gladys Pinilla-Bermúdez, Liliana Muñoz, Ariel Ivan Ruiz, Bibiana Chavarro-Portillo, Yolanda Cifuentes

• español

  Objetivo. Determinar la presencia y secuenciadel integrón clase I en un aislamiento clínicode Staphylococcus epidermidis provenientede un neonato con diagnóstico de sepsis.Materiales y métodos. En una cepa deS. epidermidis, aislada de una muestra dehemocultivo de un neonato, se realizaronlas pruebas de identificación microbiológica,susceptibilidad antimicrobiana y la caracterizaciónmolecular de los genes aac6´-aph2´´,mecA, el gen de la integrasa intI1 y el gencasete aac6´.

• English

  Objective: To determine the presence and the sequence of the class one integron in a Staphylococcus epidermidis strain isolated from a septic neonatal patient.Materials and methods: The S. epidermidis strain was isolated from a blood culture of a newborn. The microbiological identification, test of sensitivity and molecular characterization of the integrase gene int/1, the cassettegene aac6; aac6´aph2´´ resistant gene to aminoglycoside antibiotics and mecA gene resistant to ß-lactamics were realized.Results: The nucleotide sequence of the integrase gen intl1 in S. epidermidis is reported, which showed a 78% similarity to the reportedsequence of the NCBI data base in Gram negative bacteria. The gene cassette aac6 aac6´ (aminoglycoside acetilation) was also identified within the class one integron confering aminoglycoside resistance. Through molecular characterization we also found the aminoglycoside ß-lactamics resistance genes (aac6´aph2´y mecA´); the susceptibility tests showed resistance to ampicilin, oxacilin and gentamicin.Conclusions: The nucleotide sequence of the integrase gen int/1 is reported for the first time in Colombia in a S. epidermidis strain isolated from a septic neonatal patient, at the neonatal care unit of a third level hospital inBogotá. This integrase in class one integron was reported, which has shown multiresistance association in clinical isolates in Gram negative bacteria.The resistance mechanism found during this approach realized in Gram negative bacteria showed the evidence of an interspecies horizontal transfer especially by gene transfer or by moving elements such as integrons andgene cassettes.The molecular character of the causing agent of sepsis is important for epidemiological control of the infection and treatment in the newborn.