Células dendríticas y linfocitos T reguladores naturales en pacientes con enfermedad crónica de Chagas

• Autores: Paola Lasso, Adriana Cuellar, Fernando Rosas, Víctor Velasco, Concepción Puerta
• Localización: Infectio: Revista de la Asociación Colombiana de Infectología, ISSN-e 2422-3794, ISSN 0123-9392, Vol. 13, Nº. 4, 2009
• Idioma: español
• Títulos paralelos:
  • Dendritic cells and natural regulatory T lymphocytes in chronic chagasic patients
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• Resumen
  • español

    Dada la cronicidad y persistencia del parásitoTrypanosoma cruzi en la enfermedad de Chagas,el control homeostático de la respuestainmunitaria para prevenir el daño tisular ylimitar la duración del proceso inflamatorioinvolucra células con potencial reguladorcomo las células dendríticas y los linfocitos Treguladores. Es por tal motivo que el objetivode este estudio fue evaluar la proporción decélulas dendríticas totales, subpoblaciones decélulas dendríticas mieloides y plasmacitoidesy los linfocitos T reguladores naturales enpacientes con

  • English

    Given the chronicity and persistence of the parasite Trypanosoma cruzi in the Chagas disease victims, the homeostatic control of immune response to prevent tissue damage and limit the duration of the inflammatory process involves cells with regulatory potential as dendritic cells (DC) and regulatoryT cells. For this reason, the objective of this study is to evaluate the  proportion of total DC, myeloid and plasmacytoid DC subpopulations and natural regulatory T cells in chronic chagasic patients and healthy controlsubjects. Peripheral blood mononuclear cells (PBMC) from 18 chronic chagasic patients and 16 healthy control subjects were staining to myeloid DC Lin- HLA-DR+ CD11c+, plasmacytoid DC Lin-, HLA-DR+, CD123+ and natural regulatory T lymphocytes CD3+ CD4+ CD25+ Foxp3+ CD127low. Cellular proportions were calculated using the percentage obtained byflow cytometry. It was found that plasmacytoid CD proportion was lower than myeloid CD in both, chronic chagasic patients and healthy control subjects, without significant differences between the two groups. On the contrary, it was found that the proportion of natural regulatory T cells was significantlyhigher in chronic chagasic patients than in healthy control subjects upon comparing the two. These results suggest that the higher proportion of natural regulatory T cells in chronic chagasic patients might contribute to the disease pathogenesis.