Adenylate System State, Malate Dehydrogenase Activity and Expression Level in Tissues of Mytilus galloprovincialis Lamarck, 1819

• Aleksander A. Soldatov ^[1] ; Irina V. Golovina ^[1] ; Anastasia O. Lantushenko ^[2] ; Alyona S. Kokhan ^[1] ; Inna V. Sysoeva ^[1] ; Aleksander A. Sysoev ^[1] ; Evgenia E. Kolesnikova ^[1]
  1. [1] Federal Research Center «Institute of Biology of Southern Seas RAS», Moscow
  2. [2] Sevastopol State University
• Localización: Thalassas: An international journal of marine sciences, ISSN 0212-5919, Vol. 39, Nº. 2 (October 2023), 2023, págs. 739-747
• Idioma: inglés
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• Resumen

  • The activity and expression level of cytoplasmic malate dehydrogenase (MDH 1.1.1.37) gene and adenylate system state in tissues of the Mediterranean mussel Mytilus galloprovincialis Lamarck, 1819 were investigated. The highest values of adenylate energy charge (AEC) (0.66 ± 0.01) and adenylate content (ATP, ADP, and AMP) were observed in the mollusc’s foot. Close AEC values were found in the gills (0.61 ± 0.02). The lowest values were recorded in the hepatopancreas and mantle edge (AEC of 0.46–0.54) indicating moderate depression of physiological processes. A direct relationship between adenylate system state in muscle tissue and MDH activity (R2 above 0.7) was revealed, and thus this parameter can be recommended as a biomarker for energy status assessment in the mollusc’s tissue structures. A direct relationship between gene expression levels and MDH activity was shown for tissues of mussels in the range hepatopancreas → gills → foot. In contrast, in the mantle high levels of gene expression corresponded to a relatively low activity of the enzyme, presumably due to non-homogeneous tissue structure.