Study of fibroblasts activation kinetics and identification of fibroblasts subpopulations in physiological and pathological conditions
- Autores: L. Carreras, A. Martínez Blanco, Christine S. Noé, J. Alcaraz Sánchez, R. Farre
- Localización: CASEIB 2023. Libro de Actas del XLI Congreso Anual de la Sociedad Española de Ingeniería Biomédica: Contribuyendo a la salud basada en valor / coord. por Joaquín Roca González, Dolores Ojados González, Juan Suardíaz Muro, 2023, ISBN 978-84-17853-76-1, págs. 190-193
- Idioma: inglés
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Resumen
Fibroblasts undergo significant morphological and functional changes in response to specific environmental cues, adopting a novel phenotype when sustaining transformative activation in wound healing and cancerous processes. The aim of this project is to characterize cytoskeletal reorganization in both control fibroblasts’ (CFs) and cancer-associated fibroblasts’ (CAFs) activation. To achieve this, we propose a novel method based on the extraction of biophysical biomarkers from epifluorescence images of the cytoskeleton of individual fibroblasts, obtained from a patient with lung cancer and activated using TGF-β. These biophysical outputs were also used to identify CAFs subpopulations. While non-tumoral fibroblasts experience larger morphological changes characterized by an increase in area and the acquisition of a robust network of actin fibers, CAFs exhibited sustained larger areas throughout the process regardless of TGF- β administration, amongst other ...
