Preliminary screening of polycaprolactone-polystyrene electrospun scaffolds for the formation of tumoroids
- Autores: M. Pareja, E. Casanova, Joquim de Ciurana Gay, Emma Polonio Alcalá, Teresa Puig Miquel
- Localización: CASEIB 2023. Libro de Actas del XLI Congreso Anual de la Sociedad Española de Ingeniería Biomédica: Contribuyendo a la salud basada en valor / coord. por Joaquín Roca González, Dolores Ojados González, Juan Suardíaz Muro, 2023, ISBN 978-84-17853-76-1, págs. 178-181
- Idioma: inglés
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Resumen
Triple negative breast cancer (TNBC) subtype is the most aggressive and conventional chemotherapy is the standard treatment since lacks validated targeted therapies. Thus, there is an unmet need for improved in vitro research tools, including scaffolds for the formation of tumoroids. In this preliminary study, the main aim was to assess the tumoroid-forming potential of polycaprolactone (PCL)/polystyrene (PS) scaffolds. Six different polymer solutions, varying PCL/PS ratios, were formulated and PCL/PS scaffolds were manufactured using the electrospinning technique. PCL/PS structures were characterized by SEM imaging. Subsequently, a sterilization assay was performed to evaluate potential alterations derived from this process. Afterwards, TNBC MDA-MB-231 cells were cultured on PCL/PS structures for 10 days. The findings revealed that cells cultured on PCL 100/PS 0, PCL 80/PS 20, and PCL 60/PS 40 matrices exhibited the formation of tumoroids. In conclusion, ...
