New approach for determination of flumequine and oxolinic acid in aqueous samples byionic liquid-based dispersive liquid-liquid microextraction and high-performance liquid chromatography-fluorescence detection

• CARLA TOLEDO-NEIRA ^[1] ; MANUEL A BRAVO ^[2] ; ALEJANDRO ÁLVAREZ-LUEJE ^[1]
  1. [1] Universidad de Chile Facultad de Ciencias Químicas y Farmacéuticas Departamento de Química Farmacológica y Toxicológica
  2. [2] Pontificia Universidad Católica de Valparaíso Facultad de Ciencias Instituto de Química
• Localización: Journal of the Chilean Chemical Society (Boletín de la Sociedad Chilena de Química), ISSN-e 0717-6309, ISSN 0366-1644, Vol. 61, Nº. 4, 2016, págs. 3262-3266
• Idioma: inglés
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• Resumen

  • A rapid and sensitive analytical method for the determination of oxolinic acid and flumequine in aqueous samples based on dispersive liquid-liquid microextraction using ionic liquids (ILs) was developed. Based on the structural properties of the antibiotic agents studied, two ILs with different functionalities were required: ethyl-dimethyl-(2-methoxyethyl) ammonium tris(pentafluoroethyl)trifluorophosphate ([MOEDEA][FAP]) for extraction and 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BFJ) to adjust the polarity of the medium. The significant experimental factors involved in DLLME were identified and optimized using the experimental design methodology. A Plackett-Burman design was initially used for screening, and a central composite design was used for optimization. The optimized method exhibited good precision, with relative standard deviation values of less than 5 % and limits of detections on the order of 0.1 and 0.3 ng mL-1 for the two drugs. The enrichment factors for both antibiotics were 13-33 fold. The proposed method was applied to the analysis of the two antibiotic in spiked surface, river, and wastewater samples.