Antimutagenic and antioxidant potential of aqueous and acidified methanol extracts from citrus limonum fruit residues

• MUHAMMAD MUSHTAQ ^[1] ; BUSHRA SULTANA ^[1] ; FAROOQ ANWAR ^[2] ; SIDRA BATOOL ^[1]
  1. [1] University of Agriculture Department of Chemistry
  2. [2] Prince Sattam bin Abdulaziz University Department of Pharmaceutical Chemistry
• Localización: Journal of the Chilean Chemical Society (Boletín de la Sociedad Chilena de Química), ISSN-e 0717-6309, ISSN 0366-1644, Vol. 60, Nº. 2, 2015, págs. 2979-2983
• Idioma: inglés
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• Resumen

  • Aqueous and acidified methanol extracts from C. limonum fruit residues (CLFR) were evaluated for their total phenolic contents, antioxidant and antimutagenic activities. Total phenolic contents (TPC) of aqueous (30% and 70%) and acidified (0.5 N and 1.0 N) methanol extracts from CLFR were estimated by Folin-Ciocalteu reagent method whereas in-vitro antioxidant activity was assessed calorimetrically by measuring DPPH* scavenging capacity and inhibition of linoleic acid peroxidation. Anti-mutagenic potential of the extracts was appraised by Ames bacterial reverse mutation test. TPC, DPPH° scavenging capacity and inhibition of linoleic acid peroxidation were varied from 27.75-126.35 mg gallic acid equivalent (GAE)/g DW, 46-91%, and 34-83%, respectively. All the tested extracts of CLFR noticeably hunted mutagens (16.47-55.69 %) whereas none of these caused mutagenesis. Overall, acidified methanol extracts from CLFR exhibited higher extraction yields, TPC, inhibition of peroxidation and DPPH radical scavenging activity among others indicating a significant (p<0.05) variation of these attributes in relation to residue samples and extraction media. The results support that CLFR (peel and pulp biomass) being a rich source of phenolic antimutagens, can be explored as a potential candidate for the development of natural chemo-preventive drugs and nutracueticals.