Resumen de Analysis of flavonoids from leaves of acanthopanax brachypus harms
Hao-bin Hu, Xu-dong Zheng, Huai-sheng Hu
Flavonoids were extracted from the leaves of Acanthopanax brachypus Harms by microwave-assisted extraction (MAE), and four known flavonoids were isolated from the extracts by column chromatography. On the basis of physico-chemical constants and spectral data, their structures were elucidated as quercetin, quercitrin, rutin and hyperin. The influences of experimental conditions, such as solvent concentration, solution-solid ratio, microwave irradiation time and power on the extraction yields were discussed in orthogonal experiments. The four flavonoids content in the leaves was determined by HPLC analysis. The analytical results reveal that A. brachypus leaves are abundant in flavonoids, the contents of quercetin, quercitrin, rutin and hyperin are 0.86, 1.65, 2.19 and 2.68 mgg¹, respectively.
